Chris Pratt Mario (voice) Anya Taylor-Joy Princess Peach (voice) Charlie Day Luigi (voice) Jack Black Bowser (voice) Keegan-Michael Key Toad (voice) Seth Rogen Donkey Kong (voice) Fred Armisen Cranky Kong (voice) Sebastian Maniscalco Spike (voice) Charles Martinet Mario's Dad / Giuseppe (voice) Kevin Michael Richardson Kamek (voice) Khary Payton Penguin King (voice) Rino Romano Uncle Tony (voice) John DiMaggio Uncle Arthur (voice) Jessica DiCicco Mario's Mom (voice) Eric Bauza Toad General (voice) Scott Menville Koopa General (voice) Carlos Alazraqui Additional Voices (voice) Ashly Burch Additional Voices (voice) Rachel Butera Additional Voices (voice) Cathy Cavadini Additional Voices (voice) Will Collyer Additional Voices (voice) Willow Geer Additional Voices (voice) Aaron Hendry Additional Voices (voice) Andy Hirsch Additional Voices (voice) Barbara Harris Additional Voices (voice) Phil LaMarr Additional Voices (voice) Jeremy Maxwell Additional Voices (voice) Eric E. Osmond Additional Voices (voice) Noreen Reardon Additional Voices (voice) Lee Shorten Additional Voices (voice) Cree Summer Additional Voices (voice) Nisa Ward Additional Voices (voice) Nora Wyman Additional Voices (voice)